کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5800583 1555356 2014 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
New aspects in the biology of Photobacterium damselae subsp. piscicida: Pili, motility and adherence to solid surfaces
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
New aspects in the biology of Photobacterium damselae subsp. piscicida: Pili, motility and adherence to solid surfaces
چکیده انگلیسی


- HA of RBC was observed only in one Phdp strain, indicating the presence of appendages.
- We describe for the first time the presence of pilus-like structures on the surface of Phdp.
- This pathogen has been described as non-motile, but the strains tested exhibit twitching motility.
- In vitro Phdp biofilm architecture display extensive cellular chaining.

We describe for the first time the presence of pilus-like structures on the surface of Photobacterium damselae subsp. piscicida (Phdp). The hint to this discovery was the ability of one strain to hemagglutinate human erythrocytes. Further analysis of several Phdp strains ultrastructure by electron microscopy revealed the presence of long, thin fibers, similar to pili of other Gram-negative bacteria. These appendages were also observed and photographed by scanning, transmission electron microscopy and immunofluorescence. Although this fish pathogen has been described as non-motile, all strains tested exhibit twitching motility, a flagella-independent type IV-dependent form of bacterial translocation over surfaces. As far as we are aware, the movement of Phdp bacteria on semi-solid or solid surfaces has not been described previously. Moreover, we speculate that Phdp twitching motility may be involved in biofilm formation. Microscopic examination of Phdp biofilms by microscopy revealed that Phdp biofilm architecture display extensive cellular chaining and also bacterial mortality during biofilm formation in vitro. Based on our results, standardized analyses of Phdp surface appendages, biofilms, motility and their impact on Phdp survival, ecology and pathobiology are now feasible.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Veterinary Microbiology - Volume 174, Issues 1–2, 7 November 2014, Pages 247-254
نویسندگان
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