کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5800612 1555359 2014 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Genetic characterization and serological prevalence of swine hepatitis E virus in Shandong province, China
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
Genetic characterization and serological prevalence of swine hepatitis E virus in Shandong province, China
چکیده انگلیسی


- HEV genotypes were determined from a slaughterhouse in Shandong province of China.
- The HEV belonged to genotype 4, subtypes 4a and 4d.
- Swine HEV specific IgM and IgG antibodies were detected using indirect ELISAs.
- The seroprevalence of HEV IgG and IgM antibodies was 100% and 41.7% for herds.
- Genotype 4 of swine HEV infection was prevalence in Shandong province of China.

Hepatitis E virus (HEV), the causative agent of hepatitis E, is classified into four major genotypes (1 to 4) and swine is the main natural reservoir for genotypes 3 and 4. In this study, a total of 106 bile samples from a slaughterhouse in the Shandong province of China were tested for the partial ORF2 gene of HEV by RT-nPCR to determine the virus genotypes, and two indirect ELISA were developed for the detection of swine HEV specific IgM and IgG antibodies in 980 serum samples from 24 farms, in order to investigate the seroprevalence. Thirty-two out of 106 (30.2%) bile samples were positive for HEV and a high degree of partial ORF2 sequence similarity (86.8-100%) was observed among 20 samples. The viral sequences belonged to genotype 4, subtypes 4a and 4d. One complete genome sequence of a subtype 4d HEV was further determined and characterized. The seroprevalence of HEV IgG and IgM antibodies was 100% (24/24) and 41.7% (10/24) for herds, and 66.4% (651/980) and 1.6% (16/980) for the individual pigs, respectively. These results suggested a high prevalence of genotype 4 of swine HEV infection both in swine farms and at the slaughterhouse in Shandong province, which further raise public-health concerns for zoonosis and pork safety.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Veterinary Microbiology - Volume 172, Issues 3–4, 27 August 2014, Pages 415-424
نویسندگان
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