کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5800735 1555363 2014 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Laboratory diagnosis and transmissibility of bovine viral diarrhea virus from a bull with a persistent testicular infection
ترجمه فارسی عنوان
تشخیص آزمایشگاهی و انتقال ویروس اسهال ویروس گاو از گاو با عفونت بیضه مداوم
کلمات کلیدی
ویروس اسهال ویروس گاو، بول، اسپرم، عفونت حنجره
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
چکیده انگلیسی

Recently, in the United States, a dairy bull was diagnosed as the second confirmed case of persistent testicular infection (PTI) with bovine viral diarrhea virus (BVDV). The first objective of this study was to evaluate the testing methodologies currently used by the artificial insemination industry in order to improve the detection of bulls with PTI. This study evaluated the impact of multiple factors ([1] sample tested, [2] sample handling, [3] assay used, and [4] assay methodology) on the sensitivity of detection of BVDV. The second objective of this study was to evaluate the transmissibility of BVDV from the bull through casual or sexual contact. Results from this study indicate that straws of semen should be transported to the diagnostic laboratory in liquid nitrogen dry shippers. PCR proved to be a more sensitive assay than virus isolation; however, certain PCR protocols exhibited greater diagnostic sensitivity than others. Insemination with cryopreserved semen from this infected bull caused viral transmission to a seronegative heifer resulting in viremia and seroconversion. After 42 months of age, the bull appeared to clear the infection. In conclusion, this bull validates that natural exposure to a 1a strain of BVDV can result in a unique PTI causing contamination of semen with detectable infectious virus. Appropriate handling and testing of samples is necessary in order to detect bulls exhibiting PTI. Additionally, PTI with BVDV may potentially be cleared after an extended duration.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Veterinary Microbiology - Volume 170, Issues 3–4, 4 June 2014, Pages 246-257
نویسندگان
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