کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5800815 1555360 2014 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Virion-associated viral proteins of a Chinese giant salamander (Andrias davidianus) iridovirus (genus Ranavirus) and functional study of the major capsid protein (MCP)
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
Virion-associated viral proteins of a Chinese giant salamander (Andrias davidianus) iridovirus (genus Ranavirus) and functional study of the major capsid protein (MCP)
چکیده انگلیسی


- First proteomics of an amphibian-like ranavirus (ALRV) in the genus Ranavirus.
- A total of 40 CGSIV viral proteins were identified.
- Thirty-seven of 40 viral proteins are classified into three temporal kinetic classes.
- Major capsid protein was confirmed to be essential for virus production in in vitro.

Chinese giant salamander iridovirus (CGSIV) is the emerging causative agent to farmed Chinese giant salamanders in nationwide China. CGSIV is a member of the common midwife toad ranavirus (CMTV) subset of the amphibian-like ranavirus (ALRV) in the genus Ranavirus of Iridoviridae family. However, viral protein information on ALRV is lacking. In this first proteomic analysis of ALRV, 40 CGSIV viral proteins were detected from purified virus particles by liquid chromatography-tandem mass spectrometry analysis. The transcription products of all 40 identified virion proteins were confirmed by reverse transcription polymerase chain reaction analysis. Temporal expression pattern analysis combined with drug inhibition assay indicated that 37 transcripts of the 40 virion protein genes could be classified into three temporal kinetic classes, namely, 5 immediate early, 12 delayed early, and 20 late genes. The presence of major capsid proteins (MCP, ORF019L) and a proliferating cell nuclear antigen (ORF025L) was further confirmed by Western blot analysis. The functions of MCP were also determined by small interfering RNA (siRNA)-based knockdown assay and anti-recombinant MCP serum-based neutralization testing. At low dosages of CGSIV, siRNA-based knockdown of the MCP gene effectively inhibited CGSIV replication in fathead minnow cells. The antiviral effect observed in the anti-MCP serum-based neutralization test confirms the crucial function of the MCP gene in CGSIV replication. Taken together, detailed information on the virion-associated viral proteins of ALRV is presented for the first time. Our results also provide evidence that MCP is essential for CGSIV replication in vitro.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Veterinary Microbiology - Volume 172, Issues 1–2, 6 August 2014, Pages 129-139
نویسندگان
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