کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5802426 | 1555668 | 2015 | 8 صفحه PDF | دانلود رایگان |
- qRTPCR showed differential CDK gene expression in Ixodes persulcatus tissues.
- CDK10 vaccine efficacy was determined in a rodent model.
- CDK10 conferred 80% protection against I. persulcatus infestation.
Among arthropods, ticks lead as vectors of animal diseases and rank second to mosquitoes in transmitting human pathogens. Cyclin-dependent kinases (CDK) participate in cell cycle control in eukaryotes. CDKs are serine/threonine protein kinases and these catalytic subunits are activated or inactivated at specific stages of the cell cycle. To determine the potential of using CDKs as anti-tick vaccine antigens, hamsters were immunized with recombinant Ixodes persulcatus CDK10, followed by a homologous tick challenge. Though it was not exactly unexpected, IpCDK10 vaccination significantly impaired tick blood feeding and fecundity, which manifested as low engorgement weights, poor oviposition, and a reduction in 80% of hatching rates. These findings may underpin the development of more efficacious anti-tick vaccines based on the targeting of cell cycle control proteins.
Journal: Veterinary Parasitology - Volume 211, Issues 3â4, 30 July 2015, Pages 266-273