کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5821867 | 1557819 | 2015 | 8 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: The non-pathogenic Henipavirus Cedar paramyxovirus phosphoprotein has a compromised ability to target STAT1 and STAT2 The non-pathogenic Henipavirus Cedar paramyxovirus phosphoprotein has a compromised ability to target STAT1 and STAT2](/preview/png/5821867.png)
- CedPV-P shares a low degree of homology with HeV-P and NiV-P.
- The ability of CedPV-P to interact with STAT1 and STAT2 is compromised compared to HeV-P.
- Overexpression of CedPV-P does not inhibit IFN-α mediated STAT1 and STAT2 nuclear translocation.
- CedPV-P has a reduced ability to inhibit the RNA synthesis of the IFN-inducible antiviral MxA gene.
- CedPV infection is significantly less effective at reducing IFN-α induced STAT1 nuclear accumulation compared to HeV.
Immune evasion by the lethal henipaviruses, Hendra (HeV) and Nipah virus, is mediated by its interferon (IFN) antagonist P gene products, phosphoprotein (P), and the related V and W proteins, which can target the signal transducer and activator of transcription 1 (STAT1) and STAT2 proteins to inhibit IFN/STAT signaling. However, it is not clear if the recently identified non-pathogenic Henipavirus, Cedar paramyxovirus (CedPV), is also able to antagonize the STAT proteins. We performed comparative studies between the HeV P gene products (P/V/W) and CedPV-P (CedPV does not encode V or W) and demonstrate that differences exist in their ability to engage the STAT proteins using immunoprecipitation and quantitative confocal microscopic analysis. In contrast to HeV-P gene encoded proteins, the ability of CedPV-P to interact with and relocalize STAT1 or STAT2 is compromised, correlating with a reduced capacity to inhibit the mRNA synthesis of IFN-inducible gene MxA. Furthermore, infection studies with HeV and CedPV demonstrate that HeV is more potent than CedPV in inhibiting the IFN-α-mediated nuclear accumulation of STAT1. These results strongly suggest that the ability of CedPV to counteract the IFN/STAT response is compromised compared to HeV.
Journal: Antiviral Research - Volume 124, December 2015, Pages 69-76