Inhibitors of cellular kinases with broad-spectrum antiviral activity for hemorrhagic fever viruses

- A panel of inhibitors of cellular kinases was tested for their ability to inhibit multiple BSL-4 viruses.
- OSU-03012 inhibited Lassa, Ebola, Marburg and Nipah viruses.
- BIBX 1382 dihydrochloride inhibited Lassa, Ebola and Marburg viruses.
- BIBX 1382 inhibited Lassa and Ebola virus glycoprotein-dependent entry.

Host cell kinases are important for the replication of a number of hemorrhagic fever viruses. We tested a panel of kinase inhibitors for their ability to block the replication of multiple hemorrhagic fever viruses. OSU-03012 inhibited the replication of Lassa, Ebola, Marburg and Nipah viruses, whereas BIBX 1382 dihydrochloride inhibited Lassa, Ebola and Marburg viruses. BIBX 1382 blocked both Lassa and Ebola virus glycoprotein-dependent cell entry. These compounds may be used as tools to understand conserved virus-host interactions, and implicate host cell kinases that may be targets for broad spectrum therapeutic intervention.