Identification of novel virus inhibitors by influenza A virus specific reporter cell based screening

► We generated an influenza virus reporter cell line to screen a small compound library. ► Ten compounds were identified to have inhibitory activity against influenza A virus. ► Four of them blocked the activity of vRNP. ► The compound NSC 335506 inhibited HA-mediated membrane fusion. ► The reporter cell is a very useful tool to screen inhibitors against influenza A virus.