RNA interference inhibits replication of tick-borne encephalitis virus in vitro

► We developed small interfering RNAs (siRNA) targeted to the TBE virus genome. ► siRNAs reduce the quantity of TBE virus particles, genome, and protein up to 85%. ► The 50% inhibitory dose of the shRNA plasmid was 0.05 μg/ml. ► RNA interference could become valuable tool for controlling TBE virus infections.