Preparation and characterization of egg yolk immunoglobulin Y specific to influenza B virus

► Egg yolk antibody (IgY) based immunotherapy is promising in combating influenza. ► Immunized hen producing numerous inexpensive IgY antibody. ► Western blot demonstrates virus membrane protein-bonding specificity. ► Plaque reduction assay showed in vitro antiviral ability. ► Intranasal treatment resulted in significant virus clearance in challenged mice.