کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5823017 | 1117994 | 2010 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Use of preconditioned human phagocytes for extracorporeal adsorption of viruses
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ویروس شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Use of preconditioned human phagocytes for extracorporeal adsorption of viruses Use of preconditioned human phagocytes for extracorporeal adsorption of viruses](/preview/png/5823017.png)
چکیده انگلیسی
Conventional treatment of severe viral disease is limited by the narrow choice as well as the often-significant side effects or lack of clear efficacy of antiviral chemotherapy. At the same time, however, it is known that a reduction in viral load leads to significant clinical improvement in a number of important viral diseases. In this paper we discuss the possibility of using preconditioned human phagocytes in an extracorporeal biohybrid system for adsorption of viral pathogens. We present data from in vitro experiments testing adsorption of an enterovirus and of hepatitis B virus (HBV) by a preconditioned human promyelocytic cell line. While no clearance of HBV could be detected, the results revealed a near elimination of enterovirus with the cell line displaying robust viability. Enterovirus titers of 1000 (reciprocal) were reduced to a mean titer of 100.6 CCID50 with no virus detectable after adsorption in two out of five samples. Titers of 10Â 000 (reciprocal) were in turn reduced to a mean of 101.4 CCID50. The kinetics of the process was remarkable with this near elimination of the pathogen occurring within only 15Â min. Extracorporeal viral adsorption by a cellular biohybrid system appears feasible. Pairing target pathogens with suitable cell lines may offer a versatile antiviral technology.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Antiviral Research - Volume 87, Issue 2, August 2010, Pages 230-234
Journal: Antiviral Research - Volume 87, Issue 2, August 2010, Pages 230-234
نویسندگان
Felix Oberender, Heiderose Oberender, Jens Altrichter, Emil C. Reisinger, Steffen R. Mitzner,