کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5831950 | 1559753 | 2016 | 7 صفحه PDF | دانلود رایگان |
- Detergent extraction is a robust method for endotoxin removal from protein solutions; residual detergent removal challenging
- Residual detergent amounts can be quickly determined using the Endosafe⢠endotoxin detection apparatus
- The residual detergent can be effectively removed using detergent spin columns and polystyrene beads
Endotoxin removal using detergent washes and extractions are well-established, efficient, and cost-effective methods; however, removing residual detergent post treatment has been shown to be a challenge. In this communication, we show a simple and fast method for determining the detergent concentration in a protein solution post treatment and highlight strategies for detergent removal to achieve levels below the critical micelle concentration (CMC), the minimum concentration at which detergent micelles form.
Journal: International Immunopharmacology - Volume 37, August 2016, Pages 16-22