کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5832811 | 1122610 | 2014 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The chemokine-like factor 1 induces asthmatic pathological change by activating nuclear factor-κB signaling pathway
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کلمات کلیدی
CCR4MDC/CCL22CC chemokine receptor 4TARC/CCL17C19T helper type 2RPMI-1640Roswell Park Memorial Institute 1640A549IL-13IL-5CKLF1CXCR4PBMCsMDCHEK293FBSNF-κBFITCIL-4DEXWBCSDF-1PBSAsthma - آسمinterleukin 13 - اینترلوکین 13Interleukin 4 - اینترلوکین 4Interleukin 5 - اینترلوکین 5thymus and activation-regulated chemokine - تامیوز و شیمیایی تنظیم شده با فعال سازیtumor necrosis factor-alpha - تومور نکروز عامل آلفاDexamethasone - دگزامتازونSARS - سارسTARC - سبد خریدfetal bovine serum - سرم جنین گاوTH2 cells - سلول های TH2human embryonic kidney 293 cells - سلول های انسانی جنینی انسان 293peripheral blood mononuclear cells - سلول های تک هسته ای خون محیطیsevere acute respiratory syndrome - سندرم شدید حاد تنفسیstromal cell-derived factor-1 - فاکتور 1 حاصل از استروما سلولChemokine-like factor 1 - فاکتور شبه کوکین 1TNF-α - فاکتور نکروز توموری آلفاnuclear factor kappa B - فاکتور هسته ای کاپا Bphosphate buffer saline - فسفات بافر شورfluorescein isothiocyanate - فلوئورسین ایسوتیوسیاناتBronchoalveolar lavage fluid - مایع لارو برونکلوفلورMacrophage-derived chemokine - کیموکین مشتق شده از ماکروفاژwhite blood cell - گلبول سفید خونCXC chemokine receptor 4 - گیرنده 4 شیمیایی CXC
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: The chemokine-like factor 1 induces asthmatic pathological change by activating nuclear factor-κB signaling pathway The chemokine-like factor 1 induces asthmatic pathological change by activating nuclear factor-κB signaling pathway](/preview/png/5832811.png)
چکیده انگلیسی
CKLF1, which exhibits chemotactic activities on a wide spectrum of leukocytes, is up-regulated during the progress of asthma. It plays a vital role in the pathogenesis of pulmonary disease. Here, we report that CKLF1 has the capability to activate the NF-κB signaling pathway leading to the pathological change in the lung. The HEK293-CCR4 cell line, which expressed CCR4 stably, was established and screened. Western blot analysis was performed to determine the expression of NF-κB in HEK293-CCR4 and A549 cells following the C27 (10 μg/ml) added in each well at different times. These results showed that C27 (10 μg/ml) time-dependently induced the accumulation of NF-κB in the nucleus of HEK293-CCR4 and A549 cells. In addition, CKLF1 plasmid (100 μg) injection and electroporation led to the asthmatic change in the lung in mice as shown by HE and PAS staining. Furthermore, it was confirmed that CKLF1 significantly up-regulated the p-IκB expression, decreased the IκB expression, and suppressed the NF-κB expression in the cytoplasm of pulmonary tissue in vivo study. Intriguingly, an enhanced nuclear accumulation of NF-κB was observed in the lung of pCDI-CKLF1 electroporated mice, compared to that in the sham group. Therefore, the NF-κB signaling pathway was involved in the asthmatic change induced by CKLF1, among which CCR4 might play a crucial role.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Immunopharmacology - Volume 20, Issue 1, May 2014, Pages 81-88
Journal: International Immunopharmacology - Volume 20, Issue 1, May 2014, Pages 81-88
نویسندگان
Gang Li, Guang-yan Li, Zhen-zhen Wang, Hai-jie Ji, Dong-mei Wang, Jin-feng Hu, Yu-he Yuan, Gang Liu, Nai-hong Chen,