کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5838266 | 1123977 | 2012 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
In vitro and in vivo immunomodulatory activities of iridoids fraction from Barleria prionitis Linn
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کلمات کلیدی
Immunostimulant activitysRBCsWBCsmaximum tolerable doseDLCRBCsMTDDTHHBSCMIRESBarleria prionitisNBTTLCNaClHPTLCPBSRed blood cells - سلولهای قرمز خونnitroblue tetrazolium - tetrazolium nitroblueCell-mediated immunity - ایمنی سلولیdelayed-type hypersensitivity - تاخیر نوع حساسیت بالاSodium chloride - سدیم کلریدreticulo-endothelial System - سیستم رتیکولواندوتلیالphosphate buffer saline - فسفات بافر شورhigh performance thin layer chromatography - کروماتوگرافی نازک با عملکرد بالاwhite blood cells - گلبول های سفید خونsheep red blood cells - گلبول های قرمز گوسفند
موضوعات مرتبط
علوم پزشکی و سلامت
داروسازی، سم شناسی و علوم دارویی
داروشناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Ethnopharmacological relevanceBarleria prionitis Linn. (Family: Acanthaceae), one of the important Ayurvedic medicinal plant in India, has long been used to treat variety of ailments including swellings, gout, arthritic and rheumatic disorders, nervine and skin diseases, and also acts as immunorestorative.Aim of the studyThe present study was aimed to explore in vitro and in vivo immunomodulatory activities of the iridoids fraction i.e. n-butanol fraction of methanol extract from Barleria prionitis aerial parts (IFBp).Materials and methodsIFBp was studied for in vitro [nitroblue tetrazolium (NBT) test and neutrophils candidacidal assay] and in vivo immunomodulatory activity on cellular and humoral immune responses to the antigenic challenge by sheep red blood cells (SRBCs) and by neutrophil adhesion test, phagocytic activity and cyclophosphamide-induced myelosuppression. The study comprised the preliminary phytochemical screening, HPTLC standardization and maximum tolerable dose determination of IFBp.ResultsIFBp (50, 100 and 200 μg/ml) significantly (P â¤Â 0.01) increased the intracellular killing activity of stimulated neutrophils assayed by in vitro NBT reduction test and neutrophils candidacidal assay. Pretreatment of IFBp (100 and 200 mg/kg; p.o.) evoked a significant increase in percent neutrophils and neutrophils adhesion to nylon fibres. Oral administration of IFBp augmented the humoral immune response to SRBCs, evidenced by increase in antibody titres and dose dependently potentiated the delayed-type hypersensitivity reaction induced by SRBCs in mice. IFBp potentiated significantly (P â¤Â 0.01) the macrophage phagocytic activity and ameliorated the red blood cells, total white blood cells and platelets count and haemoglobin concentration, and also restored the myelosuppressive effects induced by cyclophosphamide. The content (% w/w; mean ± SD, n = 3) of main iridoids i.e. shanzhiside methyl ester and barlerin was found to be 21.55 ± 2.40 and 10.03 ± 1.69 in IFBp of BP, respectively.ConclusionThe present investigation reveals that IFBp is a potent immunostimulant, stimulating both the specific and non-specific immune mechanisms.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Ethnopharmacology - Volume 141, Issue 1, 7 May 2012, Pages 424-431
Journal: Journal of Ethnopharmacology - Volume 141, Issue 1, 7 May 2012, Pages 424-431
نویسندگان
B.V. Ghule, P.G. Yeole,