کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5839175 | 1123988 | 2012 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Aqueous extract of Centella asiatica promotes corneal epithelium wound healing in vitro
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کلمات کلیدی
PBSRCEFRIMRNaseCentella asiaticadeoxyribonucleaseRT-PCRppmGAPDHHClDNAse - DNAaseMTT - MTTEDTA - اتیلن دی آمین تترا استیک اسید RNA - اسید ریبونوکلئیکribonucleic acid - اسید ریبونوکلئیکhydrochloric acid - اسید هیدروکلریک یا اسید کلریدریک یا جوهر نمک corneal wound healing - بهبود زخم قرنیهELISA - تست الیزاNew Zealand white rabbit - خرگوش سفید نیوزیلندribonuclease - ریبونوکلئازcorneal epithelial cell - سلول اپیتلیال قرنیهphosphate buffer saline - فسفات بافر شورparts per million - قطعات در میلیونReverse transcriptase-polymerase chain reaction - واکنش زنجیره ای واکنش زنجیره ای واکنش زنجیره ایPropidium iodide - پروتئین یدیدconnexin 43 - کنکنسین 43glyceraldehyde-3-phosphate dehydrogenase - گلیسرالیدید-3-فسفات دهیدروژناز
موضوعات مرتبط
علوم پزشکی و سلامت
داروسازی، سم شناسی و علوم دارویی
داروشناسی
پیش نمایش صفحه اول مقاله
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چکیده انگلیسی
Ethnopharmacological relevanceCentella asiatica is a traditional herbal medicine that has been shown to have pharmacological effect on skin wound healing, and could be potential therapeutic agent for corneal epithelial wound healing.Aim of the studyThis study was done to evaluate the effects of Centella asiatica on the proliferation and migration of rabbit corneal epithelial (RCE) cells in the in vitro wound healing model.Materials and methodsRCE cells were cultured with or without supplementation of Centella asiatica aqueous extract. Viability and proliferation of the RCE cells was determined by MTT assay and cell cycle was analyzed by flow cytometry. In vitro re-epithelization was studied by scratch assay and migration rate was evaluated quantitatively by image analyzer. Expression of corneal specific differentiation markers, CK12 and connexin 43, were studied via RT-PCR.ResultsIt was found that supplementation of Centella asiatica did not show any significant effect on the RCE cells proliferation at the concentration up to 500 ppm, while at the concentration of 1000 ppm significantly inhibited RCE cells proliferation (p < 0.05). However, at the concentration up to 62.5 ppm, RCE cells shows significant enhancement of migration rate compared to the control group (p < 0.05). It was also found that the supplementation of Centella asiatica aqueous extract did not alter the expression of differentiation markers and cell cycle.ConclusionIn conclusion, supplementation of Centella asiatica aqueous extract at low concentrations could be useful to promote corneal epithelium wound healing.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Ethnopharmacology - Volume 140, Issue 2, 27 March 2012, Pages 333-338
Journal: Journal of Ethnopharmacology - Volume 140, Issue 2, 27 March 2012, Pages 333-338
نویسندگان
Ruszymah Bt Hj Idrus, Shiplu Roy Chowdhury, Nur Azeanty Bt Abdul Manan, Ooi Sew Fong, Mohd Ilham Adenan, Aminuddin Bin Saim,