کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5839334 1123993 2011 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Inonotus obliquus extracts suppress antigen-specific IgE production through the modulation of Th1/Th2 cytokines in ovalbumin-sensitized mice
موضوعات مرتبط
علوم پزشکی و سلامت داروسازی، سم شناسی و علوم دارویی داروشناسی
پیش نمایش صفحه اول مقاله
Inonotus obliquus extracts suppress antigen-specific IgE production through the modulation of Th1/Th2 cytokines in ovalbumin-sensitized mice
چکیده انگلیسی

Ethnopharmacological relevanceChaga mushroom (Inonotus obliquus, IO) has been used as a folk remedy for cancer, digestive system diseases, and other illnesses in Russia and Eastern Europe.Aim of the studyIn the present study, we investigated the immunomodulating effects of IO through in vivo and ex vivo studies.Materials and methodsSerum immunoglobulins (IgE, IgG1, and IgG2a) and cytokines (interleukin (IL)-4, interferon (IFN)-γ, and IL-2) were measured in concanavalin A (ConA)-stimulated splenocytes and CD4+ T cells. The nitric oxide (NO) secretion of lipopolysaccharide (LPS)-stimulated peritoneal macrophages was also measured after oral administration of 50, 100, or 200 mg kg−1 d−1 IO hot water extract (IOE) to ovalbumin (OVA)-sensitized BALB/c mice.ResultsWe found that the OVA-induced increase in serum IgE and IgG2a was significantly suppressed when IOE was orally administered after the second immunization with OVA. ConA stimulation in spleen cells isolated from OVA-sensitized mice treated with 100 mg kg−1 IOE resulted in a 25.2% decrease in IL-4 production and a 102.4% increase in IFN-γ, compared to the controls. Moreover, IL-4, IFN-γ, and IL-2 were significantly reduced after ConA stimulation in isolated CD4+T cells. We also determined that IOE inhibits the secretion of NO from LPS-stimulated peritoneal macrophages ex vivo.ConclusionsWe suggest that IO modulates immune responses through secretion of Th1/Th2 cytokines in immune cells and regulates antigen-specific antibody production.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Ethnopharmacology - Volume 137, Issue 3, 11 October 2011, Pages 1077-1082
نویسندگان
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