کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5839963 | 1124004 | 2011 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Anti-atherogenic effect of San-Huang-Xie-Xin-Tang, a traditional Chinese medicine, in cultured human aortic smooth muscle cells
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کلمات کلیدی
San-Huang-Xie-Xin-TangLPSIL-6HASMCIL-8COX-2IL-1βMCP-1ERK1/2 - ERK1 / 2MTT - MTTROS - ROSAtherosclerosis - آترواسکلروز(تصلب شریان)interleukin 1β - اینترلوکین 1βinterleukin 6 - اینترلوکین 6Interleukin 8 - اینترلوکین 8Chinese medicine - داروی چینیhuman aortic smooth muscle cell - سلول عضله صاف آئورت انسانCytokine - سیتوکینCyclooxygenase-2 - سیکلوکوکسیژناز2Lipopolysaccharides - لیپوپلی ساکارید هاMonocyte chemotactic protein-1 - پروتئین chemotactic monocyte-1Chemokine - کموکاین یا کموکین extracellular signal-regulated kinase 1/2 - کیناز 1/2 تنظیم سیگنال خارج سلولیReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم پزشکی و سلامت
داروسازی، سم شناسی و علوم دارویی
داروشناسی
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چکیده انگلیسی
Aim of the studySan-Huang-Xie-Xin-Tang (SHXXT) is a traditional Chinese medicine and it has been shown to have an anti-inflammatory effect. Since inflammation is one of the major mechanisms of atherosclerosis, we aimed to investigate anti-atherosclerotic effect of SHXXT in human aortic smooth muscle cells (HASMCs).Materials and methodsHuman aortic smooth muscle cells (HASMCs) were used in the present study, and rendered atherosclerosis by adding lipopolysaccharides. We first tested the effects of SHXXT on HASMC migration and proliferation as they present the major morphological change of atherosclerosis. We also examined whether SHXXT can influence the production of several biomarkers of inflammation and atherosclerosis including reactive oxygen species (ROS), COX-2, ERK1/2, IL-1β, IL-6, IL-8 and MCP-1.ResultsUsing the dimethyl-thiazol-diphenyltetrazoliumbromide (MTT) and wound repair assay, SHXXT was shown to significantly reduce HASMC proliferation and migration, respectively. From the fluorometric assay, SHXXT significantly reduced ROS production. SHXXT down regulated mRNA and protein levels for the COX-2 gene. In addition, phosphorylated ERK1/2 levels were suppressed by SHXXT suggesting HASMC division can be inhibited under pro-inflammatory condition. SHXXT significantly inhibited the production of IL-1β, IL-6, IL-8 and MCP-1 after LPS stimulation.ConclusionsOur results indicated that SHXXT can influence several mechanisms involved in atherosclerosis, which suggests that SHXXT may have a therapeutic potential for cardiovascular disease associated with atherosclerosis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Ethnopharmacology - Volume 133, Issue 2, 27 January 2011, Pages 442-447
Journal: Journal of Ethnopharmacology - Volume 133, Issue 2, 27 January 2011, Pages 442-447
نویسندگان
Yung-Song Wang, Ruey-Tay Lin, Hsin-Yun Cheng, Sheau-Fang Yang, Wen-Wen Chou, Suh-Hang Hank Juo,