کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5840054 | 1124004 | 2011 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Topical application of porcine placenta extract inhibits the progression of experimental contact hypersensitivity
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کلمات کلیدی
PBSDLNphorbol 12-myristate 13-acetateIL-17IL-2IFN-γDNCBIL-1βAPCIL-4CHSIL-6LPSPMA - LDC هاAntigen presenting cells - آنتیژن ارائه سلولSkin inflammation - التهاب پوستOxazolone - اگزازولونImmunomodulatory - ایمن سازیinterleukin 1 beta - اینترلوکین 1 بتاinterleukin 17 - اینترلوکین 17interleukin 2 - اینترلوکین 2Interleukin 4 - اینترلوکین 4interleukin 6 - اینترلوکین 6Contact hypersensitivity - تماس با افزایش حساسیتtumor necrosis factor alpha - تومور نکروز عامل آلفاNK cells - سلولهای NKNatural killer cells - سلولهای کشنده طبیعیantigen presenting cell - سلولهای پردازنده آنتیژنTNF-α - فاکتور نکروز توموری آلفاphosphate buffer saline - فسفات بافر شورInterferon gamma - گاما اینترفرونDraining lymph node - گره لنفاوی تخلیه
موضوعات مرتبط
علوم پزشکی و سلامت
داروسازی، سم شناسی و علوم دارویی
داروشناسی
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
Aim of studyPlacenta extract features as a composition of ointments used for skin beautification, dermatological diseases and skin dryness. However, little evidence has been cited about its underlying mechanisms of action by which it exerts a beneficial role in dermatological diseases in vivo. In this study, we intended to test the effect of topical application of porcine placenta extract in mouse model of contact hypersensitivity and elucidate its mechanism of action.Materials and methodsTo test the in vitro effect of porcine placenta extract, RAW 264.7 cells were cocultured with porcine placenta extract and stimulated with LPS (1 μg/ml) and the expression of inflammatory mediator TNF-α was estimated by RT-PCR at the mRNA level and by intracellular staining at the protein level. To further test in vivo efficacy, porcine placenta extract was topically applied to the mice with experimental skin hypersensitivity. For in vivo studies placenta extract in gel form was topically applied to ear of DNCB-induced contact hypersensitivity mouse model everyday for 2 weeks and progression of the disease was estimated by following criteria: (a) ear thickness, (b) serum IgE level by ELISA, (c) histological examination of ear tissue by H&E staining and (d) cytokine profile of total cells and CD4+ T cells by real time PCR.ResultsTopical application of porcine placenta extract on mouse ears with contact hypersensitivity decreased the severity and progression of the disease manifested by reducing ear swelling, inflammation and edema. Histological evaluation showed that placenta extract treatment reduced lymphocyte infiltration in the ear tissues. Protective effect of placenta extract is also associated with down-regulation of serum IgE level and inflammatory cytokine production (IL-1β, IFN-γ, TNF-α, IL-4, IL-12 and IL-17) in total lymph node cells and CD4+ T cells.ConclusionsOur data indicate that protective effect of porcine placenta extract in contact hypersensitivity is mediated by inhibition of the inflammatory responses and IgE production, suggesting a potential therapeutic application of porcine placenta extract to modulate skin inflammation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Ethnopharmacology - Volume 133, Issue 2, 27 January 2011, Pages 654-662
Journal: Journal of Ethnopharmacology - Volume 133, Issue 2, 27 January 2011, Pages 654-662
نویسندگان
Arijita Jash, Ho-Keun Kwon, Anupama Sahoo, Choong-Gu Lee, Jae-Seon So, Jungho Kim, Yu-Kyoung Oh, Young Bong Kim, Sin-Hyeog Im,