کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5841280 1560566 2016 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
NADPH oxidase-4 and MATER expressions in granulosa cells: Relationships with ovarian aging
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی کاردیولوژی و پزشکی قلب و عروق
پیش نمایش صفحه اول مقاله
NADPH oxidase-4 and MATER expressions in granulosa cells: Relationships with ovarian aging
چکیده انگلیسی

AimsRelevant roles in follicular development and ovulation are played by maternal antigen that embryos require (MATER), product of a maternal effect gene, and by reactive oxygen species (ROS), indispensable for the induction of ovulatory genes. At the moment, the relationship between these two biological systems and their involvement in the ovarian aging have not been still clarified. The aim of the current experimental study was to analyse the age-related changes of the MATER and NOX proteins.Materials and methodsMATER and ROS homeostasis was studied in granulosa cells (GCs) and cumulus cells (CCs) of infertile patients who undergone oocyte retrieval for in vitro fertilization cycles using Western blot and confocal immunofluorescence analysis. Samples were obtained from subjects with age ≥ 40 years (cases) and with age ≤ 37 years (controls).Key findingsThe expression pattern of MATER and NOX observed in GCs was not different from that observed in CCs. High levels of both proteins were detected in the control samples. A significant lower expression of both MATER and NOX4 was observed in the case versus control samples.SignificanceThe expression of MATER and NOX4 proteins are closely related to the follicular development and ovulation with particular regard for ovarian aging.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Life Sciences - Volume 162, 1 October 2016, Pages 108-114
نویسندگان
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