کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5841666 1560591 2015 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Isolation and characterization of cardiogenic, stem-like cardiac precursors from heart samples of patients with congenital heart disease
ترجمه فارسی عنوان
جداسازی و تعیین پیش سازهای قلبی مانند قلب مانند نمونه های قلب بیماران مبتلا به بیماری قلبی مادرزادی
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی کاردیولوژی و پزشکی قلب و عروق
چکیده انگلیسی

AimsRegenerative therapies based on resident human cardiac progenitor cells (hCPCs) are a promising alternative to medical treatments for patients with myocardial infarction. However, hCPCs are rare in human heart and finding efficient source and proper surface marker for isolation of these cells would make them a good candidate for therapy.Main methodsWe have isolated 5.34 ∗ 106 ± 2.04 ∗ 105/g viable cells from 35 heart tissue samples of 23 patients with congenital heart disease obtained during their heart surgery along with 6 samples from 3 normal subjects during cardiac biopsy.Key findingsAccording to FACS analysis, younger ages, atrial specimen and disease with increased pulmonary vascular resistance were associated with higher percentage of c-kit+ (CD117) hCPCs. Analysis for other stemness markers revealed increased CD133+ cells in the hearts of patients with congenital heart disease. By using both immune-labeling and PCR, we demonstrated that these cells express key cardiac lineage and endothelial transcription factors and structural proteins during in vitro differentiation and do express stemness transcription factors in undifferentiated state. Another novel datum of potentially relevant interest is their ability in promoting greater myocardial regeneration and better survival in rat model of myocardial infarction following transplantation.SignificanceOur results could provide evidence for conditions associated with enriched hCPCs in patients with congenital heart disease. Moreover, we showed presence of a significant number of CD133 expressing cardiogenic stem-like cardiac precursors in the heart of patients with congenital heart disease, which could be isolated and stored for future regenerative therapies in these patients.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Life Sciences - Volume 137, 15 September 2015, Pages 105-115
نویسندگان
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