کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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5841834 | 1560607 | 2015 | 6 صفحه PDF | دانلود رایگان |
AimsThe aim of this study was to investigate the effects of an active lifestyle on mitochondrial functioning, viability, bioenergetics, and redox status markers in peripheral blood mononuclear cells (PBMC) of overweight/obese postmenopausal women.Materials and methodsWe performed a cross-sectional study with postmenopausal women aged 45-64 years and body mass index > 25 kg/m2, divided into physically active (n = 23) and sedentary (n = 12) groups. Mitochondria functioning and viability, bioenergetics and redox status parameters were assessed in PBMC with spectrophotometric and fluorometric assays.Key findingsNo differences were found in the enzyme activity of complexes I and II of the electron transport chain (ETC), mitochondrial superoxide dismutase (MnSOD) activity, methyl-tetrazolium reduction levels and reduced glutathione and oxidized glutathione levels between the groups. However, the physically active group presented higher levels of reactive oxygen species (ROS) (P = 0.04) and increased catalase (CAT) (P = 0.029), total (P = 0.011) and cytosolic SOD (CuZnSOD) (P = 0.009) activities.SignificanceAn active lifestyle that includes aerobic exercise for at least 30 min, three times per week may improve antioxidant enzyme activities in PBMC in overweight/obese postmenopausal women, without changes in the activity of the ETC enzymes. However, this low intensity physical activity is not able to induce relevant mitochondrial adaptations.
Journal: Life Sciences - Volume 121, 15 January 2015, Pages 152-157