Organotypic hippocampal slice culture from the adult mouse brain: A versatile tool for translational neuropsychopharmacology

► Adult OHSC is a promising ex vivo tool for old age psychopharmacology. ► A proper method for establishing a stable adult OHSC system has been still elusive. ► We evaluated our simplified serum-free method for successful adult OHSC. ► In the serum-free culture, key features of in vivo neural tissue were well-preserved.