کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5846501 | 1128482 | 2013 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Eriodictyol-7-O-glucoside activates Nrf2 and protects against cerebral ischemic injury
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کلمات کلیدی
RT-PCRHeme oxygenase-1NQO-1AEBSFnuclear factor erythroid-2-related factor 2γ-GCSMCAOOGDEAAT3PMSFDCFH-DAHO-1NAD(P)H:quinine oxidoreductase 1Nrf2ICAGSHECA2′,7′-dichlorodihydrofluorescein diacetate - 2 '، 7'-dichlorodihydrofluorescein diacetate3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide - 3- [4،5-dimethylthiazol-2-yl] -2،5-diphenyltetrazolium bromideMTT - MTTROS - ROSAstrocyte - آستروسیتEDTA - اتیلن دی آمین تترا استیک اسید Edetic acid - اسید ایدیکmiddle cerebral artery occlusion - انسداد شریان (سرخرگ) مغزی میانیCerebral ischemia - ایسکمی مغزیBenzamidine - بنزامیدینTerminal deoxynucleotidyl transferase dUTP nick end labeling - ترمینال deoxynucleotidyl transferase dUTP نام نهایی پایان نامهTUNEL - تونلexcitatory amino acid transporter 3 - حمل کننده اسید آمینه تحریک کننده 3external carotid artery - شریان کاروتید خارجیinternal carotid artery - شریان کاروتید داخلیantioxidant response element - عنصر پاسخ آنتی اکسیدانphenylmethylsulfonyl fluoride - فنیل متیل سولفونیل فلورایدOxygen and glucose deprivation - محرومیت از اکسیژن و گلوکزARE - هستندHematoxylin and Eosin - هماتوکسیلین و ائوزینGlutathione - گلوتاتیونReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Eriodictyol-7-O-glucoside activates Nrf2 and protects against cerebral ischemic injury Eriodictyol-7-O-glucoside activates Nrf2 and protects against cerebral ischemic injury](/preview/png/5846501.png)
چکیده انگلیسی
Stroke is a complex disease that may involve oxidative stress-related pathways in its pathogenesis. The nuclear factor erythroid-2-related factor 2/antioxidant response element (Nrf2/ARE) pathway plays an important role in inducing phase II detoxifying enzymes and antioxidant proteins and thus has been considered a potential target for neuroprotection in stroke. The aim of the present study was to determine whether eriodictyol-7-O-glucoside (E7G), a novel Nrf2 activator, can protect against cerebral ischemic injury and to understand the role of the Nrf2/ARE pathway in neuroprotection. In primary cultured astrocytes, E7G increased the nuclear localization of Nrf2 and induced the expression of the Nrf2/ARE-dependent genes. Exposure of astrocytes to E7G provided protection against oxygen and glucose deprivation (OGD)-induced oxidative insult. The protective effect of E7G was abolished by RNA interference-mediated knockdown of Nrf2 expression. In vivo administration of E7G in a rat model of focal cerebral ischemia significantly reduced the amount of brain damage and ameliorated neurological deficits. These data demonstrate that activation of Nrf2/ARE signaling by E7G is directly associated with its neuroprotection against oxidative stress-induced ischemic injury and suggest that targeting the Nrf2/ARE pathway may be a promising approach for therapeutic intervention in stroke.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology and Applied Pharmacology - Volume 273, Issue 3, 15 December 2013, Pages 672-679
Journal: Toxicology and Applied Pharmacology - Volume 273, Issue 3, 15 December 2013, Pages 672-679
نویسندگان
Xu Jing, Dongmei Ren, Xinbing Wei, Huanying Shi, Xiumei Zhang, Ruth G. Perez, Haiyan Lou, Hongxiang Lou,