کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5849594 | 1561758 | 2015 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The role of Nrf2 in protection against Pb-induced oxidative stress and apoptosis in SH-SY5Y cells
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کلمات کلیدی
DPIBSOHeme oxygenase-1N-acetyl-l-cysteinePBACHBSSDCFH-DADMSAHO-1NACNQO1GCLCNrf2tert-butylhydroquinoneGCLMMFI2′7′-dichlorodihydrofluorescein diacetateMRP1γ-GCSFBSDiphenyliodonium chlorideLPSTNFt-BHQPBSkeap1 - buy1NAD(P)H:quinone oxidoreductase 1 - NAD (P) H: کینون اکسیدوردوکتاز 1ROS - ROSγ-glutamylcysteine synthetase - γ-گلوتامیل سستئین سینتاتازElectrophoretic mobility shift assay - آزمون تحرک تحرک الکتروفورزLead acetate - استات سربdimercaptosuccinic acid - اسید dimercaptosuccinicbuthionine sulfoximine - بوته یون سولفسیمیمOxidative stress - تنش اکسیداتیوLead - سربfetal bovine serum - سرم جنین گاوNeurotoxicity - سمیت عصبیEMSA یا electrophoretic mobility shift assay - سنجش تغییر تحرک الکتروفورتیکNF-E2-related factor 2 - عامل NF-E2 2antioxidant response element - عنصر پاسخ آنتی اکسیدانtumor necrosis factor - فاکتور نکروز تومورlipopolysaccharide - لیپوپلی ساکاریدPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریHank's balanced salt solution - محلول نمک متعادل هانکmean fluorescence intensity - میانگین شدت فلورسانسARE - هستندKelch-like ECH-associated protein 1 - پروتئین مرتبط با ECH کلچ 1multidrug-resistance protein - پروتئین مقاوم در برابر چندین داروAntioxidant gene - ژن آنتیاکسیدانReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش تغذیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Lead exerts severe adverse effects on the nervous system in which oxidative stress might mediate impairments. In this study, we focused on Nrf2, which has been identified to significantly influence the protection of a cellular system against many xenobiotic compounds. We found that PbAc exhibited neurotoxicity mainly through oxidant-based processes and could be inhibited by NAC and DPI in SH-SY5Y cells. As a defense response, Nrf2 was activated when exposed to PbAc, thereby inducing a rapid increase in Nrf2 nuclear accumulation, as well as Nrf2-ARE binding activities in a ROS-dependent manner. Analysis of Nrf2-regulated gene expression and protein showed that PbAc could induce the mRNA transcription of HO-1, GSTα1, GCLM, GCLC, and NQO1, as well as the protein expression of HO-1 and γ-GCS. The responses of these genes to PbAc were regulated by Nrf2. Silencing Nrf2 expression in SH-SY5Y cells inhibited PbAc-induced gene transcription and protein expression. Overexpression of Nrf2 led to decreased ROS production and cell apoptosis, as well as increased cell viability under PbAc exposure. These results indicated that the Nrf2-ARE system exhibited a protective role in Pb-induced neurotoxicity, providing potential therapeutic strategies for the prevention and treatment of Pb-related diseases.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food and Chemical Toxicology - Volume 86, December 2015, Pages 191-201
Journal: Food and Chemical Toxicology - Volume 86, December 2015, Pages 191-201
نویسندگان
Fang Ye, Xiaoyi Li, Lili Li, Ling Lyu, Jing Yuan, Jun Chen,