کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5858601 1562171 2014 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The use of optical imaging to assess the potential for embryo-fetal exposure to an exogenous material after intravaginal administration
ترجمه فارسی عنوان
استفاده از تصویربرداری نوری برای ارزیابی احتمال ابتلا به جنین به مواد خارجی پس از تزریق داخل وریدی
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست بهداشت، سم شناسی و جهش زایی
چکیده انگلیسی


- Optical imaging was used to assess embryo-fetal exposure to imaging substrates.
- Intravaginally dosed substrates rapidly appeared in the female reproductive tract.
- Intravaginally dosed substrates rapidly reached the embryo-fetus.
- Local and systemic exposures contributed to the imaging signal.

A β-actin-luc transgenic mouse model was used to evaluate whether embryo-fetal exposure could occur after intravaginal administration of a compound. A bioluminescent substrate, d-luciferin, was delivered intravaginally to mimic compound exposure to the female reproductive track and the embryo-fetus. Bioluminescence was observed throughout the reproductive tract during diestrus, but not during estrus, 2-5 min after intravaginal d-luciferin administration to female β-actin-luc mice. Intravaginal administration of d-luciferin to wild-type females mated with male β-actin-luc mice indicated that the substrate reached the developing embryo-fetus, with bioluminescence corresponding to transgene expression in the embryo-fetus. d-Luciferin substrate rapidly reached the embryo-fetus regardless of the administration route (intravaginal, intraperitoneal, subcutaneous, or intravenous). Vaginal ligation appeared to block at least some direct exposure to the embryo-fetus, but did not prevent d-luciferin from eventually reaching the embryo-fetus. Additional work will be necessary to form the basis for a reliable assessment of the human risk for male-mediated teratogenicity.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Reproductive Toxicology - Volume 48, September 2014, Pages 138-147
نویسندگان
, , , ,