کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5858937 | 1562316 | 2016 | 27 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Evaluation of 5-methylcytosine and 5-hydroxymethylcytosine as potential biomarkers for characterisation of chemical allergens
ترجمه فارسی عنوان
بررسی 5-متیل سیتوزین و 5-هیدروکسی متیل سیتوزین به عنوان بیومارکرهای بالقوه برای مشخص کردن حساسیت های شیمیایی
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کلمات کلیدی
T helper5hmCLLNATMADMRMeDIPTETDNCBAOO2,4-dinitrochlorobenzene - 2،4-dinitrochlorobenzene5mC - 5 سانتی متر5-Methylcytosine - 5-متیل سیتوزین5-hydroxymethylcytosine - 5-هیدروکسی متیل سیتوزینDNA hydroxymethylation - DNA هیدروکسی متیل شدنLocal lymph node assay - آزمایش گره لنفاوی محلیChemical allergy - آلرژی شیمیاییTrimellitic anhydride - آنیدرید تریلیتیimmunoprecipitated - ایمن سازی شدهinterferon - اینترفرونIFN - اینترفرون هاinterleukin - اینترلوکینBiomarker - بیومارکرIn vivo - درون تنی یا درونجانداریCytotoxic T cell - سلول T سیتوتوکسیکDifferentially methylated region - منطقه متخلخل متفاوتی دارد
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
چکیده انگلیسی
Epigenetic regulation of gene expression plays a pivotal role in the orchestration of immune responses. Chemical allergens form two categories: skin sensitizing chemicals associated with allergic contact dermatitis, and chemicals that cause sensitization of the respiratory tract and occupational asthma. In mice these are characterized by different T helper (Th) cell responses. Changes in DNA methylation in particular have been implicated in the in vivo responses to chemical allergy. As such it was hypothesised that differentially methylated regions (DMR) may provide candidates biomarkers of chemical allergy To examine this, mice were exposed to 2,4-dinitrochlorobenzene (DNCB; a contact allergen) or trimellitic anhydride (TMA; a respiratory allergen). DNA from draining lymph nodes was processed for methylated (5mC) and hydroxymethylated (5hmC) DNA immunoprecipitation (MeDIP/hMeDIP) then selected DMR analysed by qPCR. We describe a number of DMRs which, by combined analysis of 5mC and 5hmC, differentiate between responses induced by DNCB and those by TMA. Furthermore, these changes in methylation are specific to the draining lymph node. The Gmpr DMR is suggested as a possible biomarker for contact allergen-induced immune responses; it is characterised by divergent levels of 5mC and 5hmC DNCB-treated mice only. In contrast, the Nwc DMR was characterised by divergent 5mC and 5hmC specifically in response to TMA, highlighting its possible utility as a biomarker for responses induced by chemical respiratory allergens. These data not only represent novel analysis of 5hmC in response to chemical allergy in vivo, but with further investigation, may also provide a possible basis for differentiation between classes of chemical allergens.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology - Volume 340, 18 January 2016, Pages 17-26
Journal: Toxicology - Volume 340, 18 January 2016, Pages 17-26
نویسندگان
Victoria L. Chapman, Rémi Terranova, Jonathan G. Moggs, Ian Kimber, Rebecca J. Dearman,