کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5859891 | 1133145 | 2015 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Exposure to p,pâ²-DDE enhances differentiation of 3T3-L1 preadipocytes in a model of sub-optimal differentiation
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کلمات کلیدی
DDE3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromideSREBF1p,p′-dichlorodiphenyldichloroethyleneSlc2a4PPARG3T3-L1OROLEPT2DFABP4IBMXRT-qPCRMTT - MTTp,p′-DDE - p، p'-DDEβ-actin - β-اکتینAdipogenesis - آدیپوژنزOrganochlorine - ارگانوکلرینfatty acid synthase - اسید چرب سنتازOrganochlorine insecticides - حشره کش های آلی کلرType 2 diabetes - دیابت نوع 2Oil red O - روغن قرمز Obody mass index - شاخص توده بدنBMI - شاخص توده بدنیActb - عملFasn - فسادLeptin - لپتین Glucose transporter type 4 - نوع حمل گلوکز 4quantitative real-time polymerase chain reaction - واکنش زنجیره ای پلیمراز کمی زمان واقعی استfatty acid binding protein 4 - پروتئین اتصال دهنده اسید چرب 4sterol regulatory element-binding protein-1c - پروتئین وابسته به استرول تنظیم کننده پروتئین-1cperoxisome proliferator activated receptor-γ - گیرنده پروتئینی فعال پروکسایزوم γ
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The incidence of obesity is increasing worldwide at an alarming rate. Recently, exposure to environmental contaminants, especially organochlorines such as p,pâ²-dichlorodiphenyldichloroethylene (DDE), has been implicated as a possible causative factor in the increasing obesity epidemic. The objective of this study was to evaluate the ability of DDE to alter adipogenesis in a model of sub-optimal differentiation. 3T3-L1 preadipocytes were induced to differentiate in the presence of DDE (0.01-100 μM) using a sub-optimal differentiation cocktail. Eight days after the initiation of differentiation, adipogenesis was assessed through neutral lipid staining, triglyceride accumulation, and expression of markers of terminal differentiation. Exposure to DDE induced a concentration dependent increase in intracellular neutral lipid accumulation as determined by Oil Red O staining and triglyceride assay. Alterations in lipid accumulation were accompanied by upregulation of genetic markers of differentiation. DDE (10 μM) enhanced expression of fatty acid binding protein 4 and Sterol regulatory element-binding protein-1c at the 2.5 and 20 μM concentrations. DDE (2.5, 10, and 20 μM) induced upregulation of leptin and fatty acid synthase, as compared to sub-optimal vehicle control (0.05% ethanol). Our results indicate that DDE is capable of enhancing adipogenesis and intracellular lipid accumulation in 3T3-L1 cells through upregulation of molecular targets responsible for lipid storage.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology Letters - Volume 238, Issue 2, 14 October 2015, Pages 65-71
Journal: Toxicology Letters - Volume 238, Issue 2, 14 October 2015, Pages 65-71
نویسندگان
Lauren H. Mangum, George E. III, Janice E. Chambers,