کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5860217 | 1133171 | 2014 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Identification of the proteins related to SET-mediated hepatic cytotoxicity of trichloroethylene by proteomic analysis
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کلمات کلیدی
CBBTRISTEMEDMALDI-TOF-MS/MSDifference gel electrophoresis (DIGE)Nano-HPLCIEFTFAPMFIC50PBSDTTFBSIAATCEACNCoomassie Brilliant Blue - Coomassie درخشان آبیDMSO - DMSOIPG - GPAimmobilized pH gradient - gradient pH immobilizedsiRNA - siRNAPeptide mass fingerprinting - اثر انگشت پپتیدهAcetonitrile - استونیتریلTrifluoroacetic acid - اسید Trifluoroaceticdifference gel electrophoresis - الکتروفورز ژل تفاوتTrichloroethylene (TCE) - تریکلرتییلن (TCE)Trichloroethylene - تریکلرواتیلن isoelectric focusing - تمرکز ذره ای الکتریکیDIGE - دیگهdithiothreitol - دیتیوتریتولfetal bovine serum - سرم جنین گاوPhosphate buffered saline - فسفات بافر شورmatrix-assisted laser desorption ionization time of flight - مدت زمان یونیزاسیون لیزر جذب ماتریس پروازCHAPS - چاپسiodoacetamide - یووداکتامید
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Trichloroethylene (TCE) is an effective solvent for a variety of organic materials. Since the wide use of TCE as industrial degreasing of metals, adhesive paint and polyvinyl chloride production, TCE has turned into an environmental and occupational toxicant. Exposure to TCE could cause severe hepatotoxicity; however, the toxic mechanisms of TCE remain poorly understood. Recently, we reported that SET protein mediated TCE-induced cytotoxicity in L-02 cells. Here, we further identified the proteins related to SET-mediated hepatic cytotoxicity of TCE using the techniques of DIGE (differential gel electrophoresis) and MALDI-TOF-MS/MS. Among the 20 differential proteins identified, 8 were found to be modulated by SET in TCE-induced cytotoxicity and three of them (cofilin-1, peroxiredoxin-2 and S100-A11) were validated by Western-blot analysis. The functional analysis revealed that most of the identified SET-modulated proteins are apoptosis-associated proteins. These data indicated that these proteins may be involved in SET-mediated hepatic cytotoxicity of TCE in L-02 cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology Letters - Volume 227, Issue 1, 16 May 2014, Pages 12-19
Journal: Toxicology Letters - Volume 227, Issue 1, 16 May 2014, Pages 12-19
نویسندگان
Xiaohu Ren, Xifei Yang, Wen-xu Hong, Peiwu Huang, Yong Wang, Wei Liu, Jinbo Ye, Haiyan Huang, Xinfeng Huang, Liming Shen, Linqing Yang, Zhixiong Zhuang, Jianjun Liu,