کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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5860824 | 1133248 | 2011 | 10 صفحه PDF | دانلود رایگان |
N-nitroso compounds (NOCs) may represent a carcinogenic risk to humans following endogenous colonic nitrosation processes. We used the colon adenocarcinoma cell line Caco-2 to investigate transcriptomic changes at three time points (1, 6, 24Â h) following exposure to genotoxic concentrations of six different NOCs (two nitrosamides, four nitrosamines) with the purpose of identifying biological processes that may play a part in the carcinogenicity of these compounds. This is especially important for nitrosamide exposure where, in light of their high reactivity, important gene expression modifications may take place early in the exposure. We also analyzed NOC-induced O6-methylguanine adducts in relation to transcriptomics since these adducts may influence the expression of genes pivotal in NOC-associated carcinogenicity. Many modified pathways appeared related to DNA damage, cell cycle, apoptosis, growth factor signaling and differentiation, which are linked with carcinogenicity. Nitrosamides showed the strongest response at 1Â h of exposure, while nitrosamines had the strongest effect at 6 and 24Â h. Additionally, methylation was strongly associated with processes that may contribute to the carcinogenic risk. In summary, we have found that NOC-induced gene expression changes vary over time and that many of the modified pathways and processes indicate a carcinogenic risk associated with NOC exposure.
⺠Nitrosamines and nitrosamides differ in their temporal transcriptomic effects. ⺠Both NOC groups affect pathways related to carcinogenicity but at different times. ⺠These pathways are implicated in crucial developmental signaling cascades. ⺠NOC-induced DNA methylation also affects pathways related to carcinogenicity. ⺠The results support a carcinogenic effect of NOC exposure in the colon.
Journal: Toxicology Letters - Volume 207, Issue 3, 15 December 2011, Pages 232-241