کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5861285 | 1133757 | 2015 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Transcriptomic analysis of untreated and drug-treated differentiated HepaRG cells over a 2-week period
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کلمات کلیدی
FBSCyPPPARLFClog2 fold changeDMSO - DMSOPPAR agonists - آگونیست های PPARDimethyl sulfoxide - دیمتیل سولفواکسیدfetal bovine serum - سرم جنین گاوHepaRG cells - سلول HepaRGCytochrome P450 - سیتوکروم پی۴۵۰Transcriptomics - متن ترانهGene profiling - مشخصات ژنیperoxisome proliferator-activated receptor - گیرنده فعال فعال پروکسیوم
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Previous works have shown that differentiated human HepaRG cells can exhibit drug metabolism activities close to those of primary human hepatocytes for several weeks at confluence. The present study was designed to evaluate their long-term functional stability and their response to repeated daily drug treatments over a 14-day period, using a transcriptomic approach. Our data show that less than 1% of the expressed genes were markedly deregulated over this two weeks period and mainly included down-regulation of genes related to the cell cycle and from 3Â days, overexpression of genes involved in xenobiotic and lipid metabolism. After daily treatment with the three PPAR agonists, fenofibrate, troglitazone and rosiglitazone qualitative and/or quantitative changes in gene profiling were observed depending on the compound and duration of treatment. The highest increase in the number of deregulated genes as a function of drug treatment was seen with rosiglitazone. The most up-regulated genes common across the three compounds were mainly related to lipid and xenobiotic metabolisms. All the data support the conclusion that human HepaRG cells have an exceptional functional stability at confluence and that they are suitable for investigations on chronic effects of drugs and other chemicals.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology in Vitro - Volume 30, Issue 1, Part A, 25 December 2015, Pages 27-35
Journal: Toxicology in Vitro - Volume 30, Issue 1, Part A, 25 December 2015, Pages 27-35
نویسندگان
Camille C. Savary, Xiaoqi Jiang, Marc Aubry, Rozenn Jossé, Annette Kopp-Schneider, Philip Hewitt, André Guillouzo,