کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5861691 | 1133764 | 2014 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Gene expression responses in human lung fibroblasts exposed to alpha particle radiation
ترجمه فارسی عنوان
واکنش های بیان ژن در فیبروبلاست های ریه انسان در معرض تابش ذرات آلفا است
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کلمات کلیدی
(qRT-PCR)(ANOVA)(PBS)(FC)(FBS)(ELISA)(CT)comparative thresholdα-particles - α-ذراتAlpha - آلفاAmericium - آمریکاییEnzyme-Linked Immuno-Sorbent Assay - آنزیم مرتبط با ایمونوسیبنیتهLinear Energy Transfer - انتقال انرژی خطیGene expression - بیان ژنIngenuity Pathway Analysis - تجزیه و تحلیل راه Ingenuityanalysis of variance - تحلیل واریانسfold change - تغییر در برابرRadon - رادونRadium - رادیومfetal bovine serum - سرم جنین گاوRNA Integrity Number - شماره یکپارچه RNAPhosphate buffered saline - فسفات بافر شورRoswell Park Memorial Institute - مؤسسه یادبود پارک Roswellfalse discovery rate - میزان کشف کاذبquantitative real-time polymerase chain reaction - واکنش زنجیره ای پلیمراز کمی زمان واقعی استpolonium - پولونیوم
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
چکیده انگلیسی
This study examined alpha (α-) particle radiation effects on global changes in gene expression for the purposes of identifying potential signaling pathways that may be involved in Radon (222Rn) gas exposure and lung carcinogenesis. Human lung fibroblast cells were exposed to α-particle radiation at a dose range of 0-1.5 Gy. Twenty-four hours post-exposure, transcript modulations were monitored using microarray technology. A total of 208 genes were shown to be dose-responsive (FDR adjusted p < 0.05, Fold change > |2|) of which 32% were upregulated and 68% downregulated. Fourteen of the high expressing genes (>|4| fold) were further validated using alternate technology and among these genes, GDF15 and FGF2 were assessed at the protein level. GDF15, a known marker of lung injury, had expression levels 3-fold higher in exposed cell culture media, 24 h post-irradiation as detected by ELISA. Further, pathway analysis of the dose-responsive transcripts showed them to be involved in biological processes related to cell cycle control/mitosis, chromosome instability and cell differentiation. This panel of genes with particular focus on GDF15 may merit further analysis to determine their specific role in mechanisms leading to α-particle induced lung carcinogenesis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology in Vitro - Volume 28, Issue 7, October 2014, Pages 1222-1229
Journal: Toxicology in Vitro - Volume 28, Issue 7, October 2014, Pages 1222-1229
نویسندگان
Vinita Chauhan, Matthew Howland,