The value of selected in vitro and in silico methods to predict acute oral toxicity in a regulatory context: Results from the European Project ACuteTox

► Compounds with LD50 > 2000 mg/kg are classified correctly with moderate to good accuracy. ► Target-organ specific in vitro assays may reduce number of under-predicted toxicity. ► A revision of the current classification systems is advisable. ► Estimation of the oral dose by including kinetic parameters needs further evaluation.