In vitro biokinetics of chlorpromazine and the influence of different dose metrics on effect concentrations for cytotoxicity in Balb/c 3T3, Caco-2 and HepaRG cell cultures

► Medium concentrations decreased due to uptake into the cells. ► This decrease was dependent on the cell density and the test concentrations used. ► When the medium contains serum, the free concentration should be considered as a dose metric. ► The sensitivity ranking of the tested cell systems was dependent on the dose metric used.