کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5862246 | 1133775 | 2014 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Mechanism of an indirect effect of aqueous cigarette smoke extract on the adhesion of monocytic cells to endothelial cells in an in vitro assay revealed by transcriptomics analysis
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کلمات کلیدی
RT-qPCRIL1boxLDLVCAM-1Reverse transcriptase-quantitative polymerase chain reactionGSEAHCAECICAM-1HUVECSNrf2TNFαnESMM6FDRlog2 fold changeNFκB - NFKBAtherosclerosis - آترواسکلروز(تصلب شریان)interleukin 1 beta - اینترلوکین 1 بتاGene Set Enrichment Analysis - تجزیه و تحلیل غنی سازی مجموعه ژنیtumor necrosis factor-alpha - تومور نکروز عامل آلفاCigarette smoke - دود سیگارMicroarray - ریزآرایهHuman umbilical vein endothelial cells - سلول های اندوتلیالی ورید ناف انسانEndothelial cells - سلولهای اندوتلیالNuclear factor-kappa B - فاکتور هسته ای-کاپا BOxidized low-density lipoprotein - لیپوپروتئین با چگالی کم اکسید شدهIntercellular adhesion molecule 1 - مولکول چسبندگی بین سلولی 1Monocyte - مونوسیتfalse discovery rate - میزان کشف کاذبnormalized enrichment score - نمره غنی سازی نرمال شدهvascular cell adhesion protein 1 - پروتئین چسبندگی سلولی عروقی 1Adhesion - چسبندگی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Mechanism of an indirect effect of aqueous cigarette smoke extract on the adhesion of monocytic cells to endothelial cells in an in vitro assay revealed by transcriptomics analysis Mechanism of an indirect effect of aqueous cigarette smoke extract on the adhesion of monocytic cells to endothelial cells in an in vitro assay revealed by transcriptomics analysis](/preview/png/5862246.png)
چکیده انگلیسی
The adhesion of monocytic cells to the “dysfunctional” endothelium constitutes a critical step in the initiation of atherosclerosis. Cigarette smoke (CS) has been shown to contribute to this process, the complex mechanism of which still needs to be unraveled. We developed an in vitro adhesion assay to investigate the CS-induced adhesion of monocytic MM6 cells to human umbilical vein endothelial cells (HUVECs) following exposure to an aqueous CS extract (smoke-bubbled phosphate buffered saline: sbPBS), reasoning that in vivo monocytes and endothelial cells are exposed primarily to soluble constituents from inhaled CS absorbed through the lung alveolar wall. MM6 cell adhesion was increased exclusively by the conditioned medium from sbPBS-exposed MM6 cells, not by direct sbPBS exposure of the HUVECs within a range of sbPBS doses. Using a transcriptomics approach followed by confirmation experiments, we identified different exposure effects on both cell types and a key mechanism by which sbPBS promoted the adhesion of MM6 cells to HUVECs. While sbPBS provoked a strong oxidative stress response in both cell types, the expression of E-selectin, VCAM-1 and ICAM-1, responsible for the adhesion of MM6 cells to HUVECs, was induced in the latter through a proinflammatory paracrine effect. We confirmed that this effect was driven mainly by TNFα produced by MM6 cells exposed to sbPBS. In conclusion, we have elucidated an indirect mechanism by which sbPBS increases the adhesion of monocytic cells to endothelial cells in this in vitro assay that was designed for tobacco product risk assessment while mimicking the in vivo exposure conditions as closely as possible.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology in Vitro - Volume 28, Issue 5, August 2014, Pages 896-908
Journal: Toxicology in Vitro - Volume 28, Issue 5, August 2014, Pages 896-908
نویسندگان
Carine Poussin, Inka Gallitz, Walter K. Schlage, Yvonne Steffen, Katrin Stolle, Stefan Lebrun, Julia Hoeng, Manual C. Peitsch, Michael Lietz,