کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5862370 1133778 2013 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Effect of oxygen concentration and selected protocol factors on viability and gene expression of mouse liver slices
ترجمه فارسی عنوان
اثر غلظت اکسیژن و عوامل پروتکل انتخاب شده بر روی قابلیت حیاتی و بیان ژن کراکرهای ماوس
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست بهداشت، سم شناسی و جهش زایی
چکیده انگلیسی


- We aimed to characterize and to optimize mouse liver slices (PCLSs) culture.
- We investigated effects of oxygen concentration and selected protocol factors.
- Biochemical and transcriptomic analyses were performed.
- PCLS retain many features of the liver in vivo.
- About 80% of oxygen yields slices with the best characteristics.

Precision cut liver slices (PCLSs) are widely used as a model to study hepatotoxicity. For culturing of PCLS diverse protocols are used which could affect slices viability and results.We aimed to identify the most optimal culture protocol for mouse PCLS. Slices were cultured for 24 h under different concentrations of serum, glucose, insulin, and oxygen. Thereafter, slices viability was assessed by biochemical methods. Transcriptome analysis was performed to identify changes introduced by culture at different oxygen concentrations (20%, 40%, 60%, and 80% of oxygen).Medium composition did not affect the slices viability. Although metabolic competence was unaffected by oxygen concentrations, culturing at 80% of oxygen yielded slices with the best biochemical characteristics. The comparison of uncultured vs. cultured slices revealed 2524 genes to be differentially expressed. Genes involved in drug metabolism, peroxisomal and mitochondrial functions were down-regulated while several adaptive/stress response processes were up-regulated. Moreover, 80% of oxygen was the most favorable condition with respect to maintenance of expression of genes involved in drug and energy metabolism.The outcome of this study indicates that mouse PCLS are a valuable tool in research on hepatic functions and toxicity, particularly if they are cultured under a controlled oxygen concentration of 80%.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology in Vitro - Volume 27, Issue 5, August 2013, Pages 1513-1524
نویسندگان
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