کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5862453 1133780 2013 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Tigecycline prevents LPS-induced release of pro-inflammatory and apoptotic mediators in neuronal cells
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
Tigecycline prevents LPS-induced release of pro-inflammatory and apoptotic mediators in neuronal cells
چکیده انگلیسی

Pro-inflammatory and pro-apoptotic mediators have been involved in the pathogenesis of neurodegenerative diseases. Tigecycline (Tig), a glycylcycline antibiotic and an analog of Minocycline, is shown to exert anti-inflammatory effects that are distinct from its anti-microbial activity. Its neuroprotective mechanism is unknown. In this study, we investigated the direct protective mechanisms of tigecycline against lipopolysaccharide (LPS)-induced Rat pheochromocytoma (PC12) cells. The results showed that tigecycline significantly attenuated the expression and the release of nuclear factor-kappa beta (NF-κB), tumor necrosis factor-alpha (TNF-α) and interleukin-1beta (IL-1β), as well as nitric oxide (NO) levels in LPS-induced PC12 cells. In addition, tigecycline dose-dependently decreased cytochrome c release and caspase-3 activity. This later finding corroborated the results of decreased pro-apoptotic Bad, and increased anti-apoptotic Bcl-2 protein expression thus, confirming a neuroprotective effect of the drug in differentiated PC12 cells induced with LPS. The findings of our study suggest new targets for tigecycline and support the potential for tigecycline to be investigated as a therapeutic agent for neurodegenerative disorders.

► Tigecycline protects PC12 cells against LPS cytotoxicity. ► Tigecycline reduced NF-κB expression in LPS-induced neuronal cells. ► Tigecycline decreased LPS-induced cytokines expression and release. ► Tigecycline inhibited apoptotic mediators in LPS-induced PC12 cells.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology in Vitro - Volume 27, Issue 2, March 2013, Pages 686-693
نویسندگان
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