A comparative performance test of standard, medium- and high-throughput comet assays

► Standard, medium and high throughput versions of the comet assay compared in two independent laboratories. ► Measurement of MMS and X-ray induced DNA damage. ► As many as 384 comet assay gels analysed in one electrophoresis run. ► Standard, medium and high throughput comet assays give identical estimates of DNA damage.