Titanium dioxide nanoparticles induced cytotoxicity, oxidative stress and DNA damage in human amnion epithelial (WISH) cells

► Human amniotic (WISH) cells exposed to TiO2-NPs demonstrated cytotoxic response. ► TiO2-NPs treated cells exhibited decline in catalase and glutathione enzymes. ► The treated cells show a significant increase in intracellular ROS generation. ► TiO2-NPs treated cells also demonstrated the formation of DNA double strand breaks. ► With increasing concentration of TiO2-NPs, WISH cells showed G2/M cell cycle arrest.