کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5863094 | 1133793 | 2012 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Comet assay analysis of DNA damage in T- and B-lymphocytes separated by MACS for human biomonitoring studies
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
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چکیده انگلیسی
In this study, we developed and tested a method for human biomonitoring using Comet assays with human T- and B-lymphocytes obtained by magnetic cell sorting (MACS). We evaluated DNA damage induced by treatment with hydrogen peroxide (H2O2; 5, 25 and 50 μM) and methyl methane sulfonate (MMS; 5, 25 and 50 μM) in both human B- and T-lymphocytes obtained by MACS, and compared their DNA damage levels. Significant, dose-dependent levels of DNA damage were found in T-lymphocytes and B-lymphocytes. Furthermore, the level of DNA damage was significantly greater in B-lymphocytes than in T-lymphocytes, suggesting that human B-lymphocytes may be a more sensitive target than T-lymphocytes for the evaluation of DNA damage. In addition, we compared these in vitro exposure data with previous studies that showed DNA damage in B- and T-lymphocyte and granulocytes of control subjects and industrial workers exposed in vivo to environmental toxicants. The use of single types of human peripheral blood mononuclear cells obtained by MACS, for Comet assays gave sensitive and reliable data for human biomonitoring for environmental toxicants.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology in Vitro - Volume 26, Issue 2, March 2012, Pages 369-372
Journal: Toxicology in Vitro - Volume 26, Issue 2, March 2012, Pages 369-372
نویسندگان
So-Young Park, Eunkyung Cho, Eunha Oh, Donggeun Sul,