کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5889050 | 1568133 | 2016 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Moderate chronic kidney disease impairs bone quality in C57Bl/6J mice
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کلمات کلیدی
ImaxTb.NCOL1A1Ct.PoCt.ThTMDCKD-MBDALPLtrabecular bone volume fractionpTHCminConn.DBglapIBSPMARperiosteal perimeterthyroparathyroidectomyBV/TV - BV / TVchronic kidney disease - بیماری مزمن کلیویkidney disease - بیماری کلیویcortical porosity - تخلخل کرویconnectivity density - تراکم اتصالBUN - خوبNanoindentation - دندانه گذاری نانوtrabecular number - شماره تراککالtrabecular thickness - ضخامت تراککالcortical thickness - ضخامت قشرCortical bone area - منطقه استخوان کورتیکcortical area - منطقه قشرMineral apposition rate - میزان جذب مواد معدنیCKD - نارسایی مزمن کلیهblood urea nitrogen - نیتروژن اوره خونparathyroid hormone - هورمون پاراتیروئیدPMMA - پلی (متیل متاکریلات) Bone quality - کیفیت استخوان
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شناسی تکاملی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Chronic kidney disease (CKD) increases bone fracture risk. While the causes of bone fragility in CKD are not clear, the disrupted mineral homeostasis inherent to CKD may cause material quality changes to bone tissue. In this study, 11-week-old male C57Bl/6J mice underwent either 5/6th nephrectomy (5/6 Nx) or sham surgeries. Mice were fed a normal chow diet and euthanized 11 weeks post-surgery. Moderate CKD with high bone turnover was established in the 5/6 Nx group as determined through serum chemistry and bone gene expression assays. We compared nanoindentation modulus and mineral volume fraction (assessed through quantitative backscattered scanning electron microscopy) at matched sites in arrays placed on the cortical bone of the tibia mid-diaphysis. Trabecular and cortical bone microarchitecture and whole bone strength were also evaluated. We found that moderate CKD minimally affected bone microarchitecture and did not influence whole bone strength. Meanwhile, bone material quality decreased with CKD; a pattern of altered tissue maturation was observed with 5/6 Nx whereby the newest 60 μm of bone tissue adjacent to the periosteal surface had lower indentation modulus and mineral volume fraction than more interior, older bone. The variance of modulus and mineral volume fraction was also altered following 5/6 Nx, implying that tissue-scale heterogeneity may be negatively affected by CKD. The observed lower bone material quality may play a role in the decreased fracture resistance that is clinically associated with human CKD.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bone - Volume 86, May 2016, Pages 1-9
Journal: Bone - Volume 86, May 2016, Pages 1-9
نویسندگان
Chelsea M. Heveran, Alicia M. Ortega, Andrew Cureton, Ryan Clark, Eric W. Livingston, Ted A. Bateman, Moshe Levi, Karen B. King, Virginia L. Ferguson,