کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5889309 | 1568138 | 2015 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Pregnancy-associated plasma protein-A modulates the anabolic effects of parathyroid hormone in mouse bone
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کلمات کلیدی
BMCBMDIGF-IpQCTpTHPAPP-ADEXAIGFBPIGF-IRBone mineral density - تراکم معدنی استخوانdual energy X-ray absorptiometry - جذب سنجی اشعه ایکس انرژی دوگانهInsulin-like growth factor-I - عامل رشد انسولین مانند IInsulin-like growth factor - فاکتور رشد مانند انسولینBone mineral content - محتوای مواد معدنی استخوانknock-out - ناک اوتwild-type - نوع وحشیparathyroid hormone - هورمون پاراتیروئیدpregnancy-associated plasma protein-A - پروتئین A-پلاسمای مرتبط با بارداریinsulin-like growth factor binding protein - پروتئین اتصال دهنده به عامل انسولین مانندinsulin-like growth factor binding proteins - پروتئین های اتصال دهنده عامل رشد انسولین مانندIGF-I receptor - گیرنده IGF-I
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شناسی تکاملی
پیش نمایش صفحه اول مقاله
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چکیده انگلیسی
Intermittent parathyroid hormone (PTH) is a potent anabolic therapy for bone, and several studies have implicated local insulin-like growth factor (IGF) signaling in mediating this effect. The IGF system is complex and includes ligands and receptors, as well as IGF binding proteins (IGFBPs) and IGFBP proteases. Pregnancy-associated plasma protein-A (PAPP-A) is a metalloprotease expressed by osteoblasts in vitro that has been shown to enhance local IGF action through cleavage of inhibitory IGFBP-4. This study was set up to test two specific hypotheses: 1) Intermittent PTH treatment increases the expression of IGF-I, IGFBP-4 and PAPP-A in bone in vivo, thereby increasing local IGF activity. 2) In the absence of PAPP-A, local IGF activity and the anabolic effects of PTH on bone are reduced. Wild-type (WT) and PAPP-A knock-out (KO) mice were treated with 80 μg/kg human PTH 1-34 or vehicle by subcutaneous injection five days per week for six weeks. IGF-I, IGFBP-4 and PAPP-A mRNA expression in bone were significantly increased in response to PTH treatment. PTH treatment of WT mice, but not PAPP-A KO mice, significantly increased expression of an IGF-responsive gene. Bone mineral density (BMD), as measured by DEXA, was significantly decreased in femurs of PAPP-A KO compared to WT mice with PTH treatment. Volumetric BMD, as measured by pQCT, was significantly decreased in femoral midshaft (primarily cortical bone), but not metaphysis (primarily trabecular bone), of PAPP-A KO compared to WT mice with PTH treatment. These data suggest that stimulation of PAPP-A expression by intermittent PTH treatment contributes to PTH bone anabolism in mice.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bone - Volume 81, December 2015, Pages 413-416
Journal: Bone - Volume 81, December 2015, Pages 413-416
نویسندگان
Kari B. Clifton, Cheryl A. Conover,