کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5889351 | 1568138 | 2015 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Knee loading protects against osteonecrosis of the femoral head by enhancing vessel remodeling and bone healing
ترجمه فارسی عنوان
بارگذاری زانو در برابر استئونکروز سر ران با محافظت در برابر تغییر ساختار و بهبود استخوان محافظت می کند
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کلمات کلیدی
BMCRANKLBMDTRAPvessel remodelingONFHM-CSFOsteonecrosis of the femoral head - Osteonecrosis سر فمورالALP - آلکالن فسفاتازAlkaline phosphatase - آلکالین فسفاتاز یا فسفاتاز قلیاییOsteoblast - استئوبلاست osteonecrosis - استئونکروزOsteoclast - استخوانکاه، استئوکلاستTartrate resistant acid phosphatase - اسید فسفاتاز مقاوم در برابر تتراتKnee loading - بارگذاری زانوbone remodeling - بازسازی استخوانBone mineral density - تراکم معدنی استخوانmacrophage-colony stimulating factor - ماکروفاژ - کلنی عامل تحریک کنندهBone mineral content - محتوای مواد معدنی استخوانreceptor activator of nuclear factor kappa-B ligand - گیرنده گیرنده لیگاند کاپا-B فاکتور هسته ای
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شناسی تکاملی
چکیده انگلیسی
Osteonecrosis of the femoral head is a serious orthopedic problem. Moderate loads with knee loading promote bone formation, but their effects on osteonecrosis have not been investigated. Using a rat model, we examined a hypothesis that knee loading enhances vessel remodeling and bone healing through the modulation of the fate of bone marrow-derived cells. In this study, osteonecrosis was induced by transecting the ligamentum teres followed by a tight ligature around the femoral neck. For knee loading, 5 N loads were laterally applied to the knee at 15 Hz for 5 min/day for 5 weeks. Changes in bone mineral density (BMD) and bone mineral content (BMC) of the femur were measured by pDEXA, and ink infusion was performed to evaluate vessel remodeling. Femoral heads were harvested for histomorphometry, and bone marrow-derived cells were isolated to examine osteoclast development and osteoblast differentiation. The results showed that osteonecrosis significantly induced bone loss, and knee loading stimulated both vessel remodeling and bone healing. The osteonecrosis group exhibited the lowest trabecular BV/TV (p < 0.001) in the femoral head, and lowest femoral BMD and BMC (both p < 0.01). However, knee loading increased trabecular BV/TV (p < 0.05) as well as BMD (p < 0.05) and BMC (p < 0.01). Osteonecrosis decreased the vessel volume (p < 0.001), vessel number (p < 0.001) and VEGF expression (p < 0.01), and knee loading increased them (p < 0.001, p < 0.001 and p < 0.01). Osteonecrosis activated osteoclast development, and knee loading reduced its formation, migration, adhesion and the level of “pit” formation (p < 0.001, p < 0.01, p < 0.001 and p < 0.001). Furthermore, knee loading significantly increased osteoblast differentiation and CFU-F (both p < 0.001). A significantly positive correlation was observed between vessel remodeling and bone healing (both p < 0.01). These results indicate that knee loading could be effective in repair osteonecrosis of the femoral head in a rat model. This effect might be attributed to promoting vessel remodeling, suppressing osteoclast development, and increasing osteoblast and fibroblast differentiation. In summary, the current study suggests that knee loading might potentially be employed as a non-invasive therapy for osteonecrosis of the femoral head.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bone - Volume 81, December 2015, Pages 620-631
Journal: Bone - Volume 81, December 2015, Pages 620-631
نویسندگان
Daquan Liu, Xinle Li, Jie Li, Jing Yang, Hiroki Yokota, Ping Zhang,