High glucose concentrations alter the biomineralization process in human osteoblastic cells

► High-glucose alters biomineralization process in osteoblastic cells. ► High-glucose raises the amount, but diminishes the quality of mineral deposited. ► High glucose increases RANKL, osteocalcin, BSP and Cbfa and decreases OPG expression. ► High-glucose increases the expression of IL1beta, IL6, IL8, MCP-1 and IL10 mRNAs. ► Expression of TLR-2, -3 and -4 in osteoblastic cells seems susceptible to osmotic stress.