کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5904553 | 1158009 | 2012 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Ascorbic acid alleviates toxicity of paclitaxel without interfering with the anticancer efficacy in mice
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
ALTbFGFTMAPBSCeA - CEAH&E - H & EROS - ROSAST - آسپارتات ترانس آمینازAscorbic acid - آسکوربیک اسیدalanine transaminase - آلانین ترانس آمینازcarcinoembryonic antigen - آنتی ژن carcinoembryonicaspartate transaminase - ترانس آمیناز آسپارتاتBUN - خوبCytotoxicity - سمیت سلولیVascular endothelial growth factor - فاکتور رشد اندوتلیال عروقیVascular Endothelial Growth Factor (VEGF) - فاکتور رشد اندوتلیال عروقی (VEGF)basic fibroblast growth factor - فاکتور رشد فیبروبلاست پایهPhosphate buffered saline - فسفات بافر شورTissue microarray - میکروآرشی بافتblood urea nitrogen - نیتروژن اوره خونHematoxylin and Eosin - هماتوکسیلین و ائوزینpolymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمرازPaclitaxel - پاکلی تاکسلnegative control - کنترل منفیReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
علوم غدد
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Paclitaxel is used extensively as a chemotherapeutic agent against a broad range of tumors but often leads to the early termination of treatment due to severe toxic side effects. In this study, we hypothesized that ascorbic acid could reduce the toxic side effects without interfering with the anticancer effect of paclitaxel. To demonstrate this, we examined the effect of the combinational treatment of ascorbic acid and paclitaxel using H1299 (a non-small cell lung cancer cell line) and BALB/c mice implanted with or without sarcoma 180 cancer cells. In H1299 cells, the anticancer effects of the combinational treatment with paclitaxel and ascorbic acid were up to 1.7-foldhigher than those of single-agent paclitaxel treatment. In addition, it was shown that the viability of the HEL299 normal cells was up to 1.6-fold higher with the combinational treatment than with paclitaxel treatment alone. In vivo mouse experiments also showed that mice co-treated with paclitaxel and ascorbic acid did not exhibit the typical side effects induced by paclitaxel, such as a reduction in the numbers of white blood cells and red blood cells and the level of hemoglobin (P < .05). The analysis of cancer-related gene expression by quantitative real-time polymerase chain reaction and immunohistochemistry revealed that the combinational treatment suppressed cancer cell multiplication. Taken together, these results suggest that combinational chemotherapy with ascorbic acid and paclitaxel not only does not block the anticancer effects of paclitaxel but also alleviates the cytotoxicity of paclitaxel in vivo and in vitro.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Nutrition Research - Volume 32, Issue 11, November 2012, Pages 873-883
Journal: Nutrition Research - Volume 32, Issue 11, November 2012, Pages 873-883
نویسندگان
Jin-Hee Park, Keith R. Davis, Gunsup Lee, Manyong Jung, Yuchul Jung, Jungan Park, Sang-Yeop Yi, Myung-Ah Lee, Sukchan Lee, Chang-Hwan Yeom, Jin Kim,