کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5905275 | 1159870 | 2015 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Functional conservation and divergence of duplicated fibroblast growth factor receptor 1 (fgfr1) genes in blunt snout bream (Megalobrama amblycephala)
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کلمات کلیدی
ORFPKCMS-222hpfqRT-PCRFGFFGFR1HSPGMegalobrama amblycephalaPBSPI3KERKs - ERK هاhGH - HGHMAPKs - MAPK هاMHb - MHBRTKs - RTK هاtricaine methanesulfonate - تری کینین متانسولفوناتdigoxigenin - دیگوکسین ژنhour post-fertilization - ساعت پس از لقاحDIG - شماfibroblast growth factor - فاکتور رشد فیبروبلاستphosphoinositide 3-kinase - فسفینوزیتید 3-کینازopen reading frame - قاب خواندن بازPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریUTR یا untranslated regions - منطقه ترجمه نشدهuntranslated region - منطقه غیر ترجمهHuman growth hormone - هورمون رشد انسانHeparan sulfate proteoglycan - هپارین سولفات پروتئگلیکانquantitative real-time PCR - واکنش زنجیره ای پلیمراز واقعی در زمان واقعیProtein kinase C - پروتئین کیناز سیDuplicated genes - ژن های تکثیر شدهextracellular signal-regulated kinases - کیناز های تنظیم شده سیگنال خارج سلولیmitogen-activated protein kinases - کیناز پروتئین فعال Mitogenreceptor tyrosine kinases - گیرنده تیروزین کینازfibroblast growth factor receptor - گیرنده فاکتور رشد فیبروبلاست
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Fgfr1 is a fibroblast growth factor receptor involved in regulating cell growth, proliferation, differentiation and migration. Here, we report the isolation and characterization of duplicated fgfr1 genes in blunt snout bream (Megalobrama amblycephala). Blunt snout bream fgfr1a and -1b cDNAs were found to share a relatively high sequence identity of 82%. During embryogenesis, both fgfr1a and -1b mRNAs were highly detected at zygotes but gradually decreased and then constantly expressed after 16Â hpf, besides a strong expression for the fgfr1b mRNA at 12Â hpf. Whole-mount in situ hybridization demonstrated that fgfr1a mRNA was transcribed at the eyes, mid-hindbrain boundary (MHB), brain, posterior somites and tailbud at 16Â hpf, while the fgfr1b mRNA was only detected at the eyes and posterior somites at the same period. At 28Â hpf embryos, both fgfr1a and -1b mRNAs were expressed in the eyes, brain, pharyngeal arches and tailbud, and in the eyes, brain, pharyngeal arches and notochord at 55Â hpf. In adult fish, fgfr1a mRNA was strongly expressed in the gill, gonad, brain and midgut, but examined relatively low in the skin and kidney. In contrast, the fgfr1b mRNA was highly detected in the brain and liver and quite low in the skin, gill and kidney. During starvation, both fgfr1a and -1b mRNAs were significantly up-regulated in the intestine and liver, but down-regulated in the brain. Moreover, duplicated fgfr1 mRNAs were differentially inhibited in tissues with exogenous recombinant hGH. Our results suggest that two fgfr1 genes play important roles in regulating growth and development in blunt snout bream.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 573, Issue 2, 1 December 2015, Pages 225-232
Journal: Gene - Volume 573, Issue 2, 1 December 2015, Pages 225-232
نویسندگان
Qian-Qian Zhang, Fu-Gui Li, Bo Qin, Jie Chen, Xia-Yun Jiang, Shu-Ming Zou,