کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5905561 | 1159908 | 2015 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
MicroRNA biogenesis pathway from the salmon louse (Caligus rogercresseyi): Emerging role in delousing drug response
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کلمات کلیدی
Caligus rogercresseyiAGO1dsRNAAZApri-miRNARPKMargonaute2AzamethiphosPACTCnot7Exportin-5RNA-seqNCBIPABPTSAdsRNA-binding motifArgonaute1Primary miRNA - miRNA اولیهRNA interference - RNA تداخل کنندهdouble-stranded RNA - RNA دو رشته ایRNAi - RNA سرکوبگر،RNA مداخلهگر، RNA خاموش کنندهArgonaute - آرگونوتAgo2 - اکشن 2post-transcriptional gene silencing - خاموش کردن ژن post-transcriptionalRISC - خطرDelta - دلتاDeltamethrin - دلتامترینSea lice - شپش دریاییNon-synonymous - غیر مترادفRNA-Induced Silencing Complex - مجتمع خاموش کننده RNA inducedNational Center for Biotechnology Information - مرکز ملی اطلاعات بیوتکنولوژیTranscriptome shotgun assembly - مونتاژ تفنگ ساچمه ایMicroRNA - میکرو RNA MiRNA - میکروRNA، ریزآرانای، miRNAPoly(A)-binding protein - پروتئین Poly (A) اتصالSingle nucleotide polymorphism - پلیمورفیسم تک نوکلئوتیدیAGO - پیشSNP - چندریختی تک-نوکلئوتید
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
Despite the increasing evidence of the importance of microRNAs (miRNAs) in the regulation of multiple biological processes, the molecular bases supporting this regulation are still barely understood in crustaceans. Therefore, the molecular characterization and transcriptome modulation of the miRNA biogenesis pathway were evaluated in the salmon louse Caligus rogercresseyi, an ectoparasite that constitutes one of the biggest concerns for salmonid aquaculture industry. Hence, RNA-Seq analysis was conducted from six different developmental stages, and also after bioassays with delousing drugs Deltamethrin and Azamethiphos using adult individuals. In silico analysis evidenced 24 putative genes involved in the miRNA pathway such as biogenesis, transport, maturation and miRNA-target interaction. Moreover, 243 putative single nucleotide polymorphisms (SNPs) were identified, 15 of which showed non-synonym mutations. RNA-Seq analysis revealed that CCR4-Not complex subunit 3 (CNOT3) was upregulated at earlier developmental stages (nauplius I-II and copepodid), and also after the exposure to Azamethiphos, but not to Deltamethrin. In contrast, the subunit 7 (CNOT7) showed an inverse expression pattern. Different Argonaute transcripts were associated to chalimus and adult stages, revealing specific expression patterns in response to antiparasitic drugs. Our results suggest novel insights into the regulatory network of the post-transcriptional gene regulation in C. rogercresseyi mediated by miRNAs, evidencing a putative role during the ontogeny and drug response.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 555, Issue 2, 25 January 2015, Pages 231-241
Journal: Gene - Volume 555, Issue 2, 25 January 2015, Pages 231-241
نویسندگان
Diego Valenzuela-Miranda, Gustavo Nuñez-Acuña, Valentina Valenzuela-Muñoz, Sassan Asgari, Cristian Gallardo-Escárate,