کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5905566 | 1159908 | 2015 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Identification, phylogenetic evolutionary analysis of GDQY orf virus isolated from Qingyuan City, Guangdong Province, southern China
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کلمات کلیدی
bovine papular stomatitis virusPseudocowpox virusCPEORFVMOI - MEcytopathic effects - اثرات سیتوپاتیکContagious ecthyma - اکتیو مضرTem - این استGoat - بزphylogenetic analysis - تجزیه و تحلیل فیلوژنتیکmolecular evolutionary genetics analysis - تجزیه و تحلیل ژنتیک تکامل مولکولیGenetic diversity - تنوع ژنتیکیSouthern China - جنوب چینminimum essential medium - حداقل حداقل مورد نیازMEM - مامانMEGA - مگاTransmission electron microscopy - میکروسکوپ الکترونی عبوریpolymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمرازorf virus - ویروس ویروسmultiplicity of infection - چندین عفونتKilovolt - کیولولت
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Infection with the orf virus (ORFV) leads to contagious ecthyma, also called contagious pustular dermatitis, which usually affects sheep, goats and other small ruminants. It has a great distribution throughout the world and has also been reported to infect humans. Though many strains have been isolated from differing parts of mainland China, rarely has any strain been reported from the southern provinces of China. We studied a case of orf virus infection that occurred at Qingyuan City, Guangdong Province in southern China. An orf virus strain, GDQY, was successfully isolated and identified through cell culture techniques and transmission electron microscopy. Complete genes of ORFV011, ORFV059, ORFV106 and ORFV107 were amplified for the sequence analysis based on their nucleotide or amino acid level. In order to discuss the genetic variation, precise sequences were used to compare to other reference strains isolated from different districts or countries. Phylogenetic trees based on those strains were built up and evolutionary distances were calculated based on the alignment of their complete sequences. The typical structure of the orf virus was observed in cell-culture suspensions inoculated with GDQY, and the full-length of four genes was amplified and sequenced. Phylogenetic analysis indicated that GDQY is homologous to FJ-DS and CQ/WZ on ORFV011 nucleotides. ORFV059 may be more variable than ORFV011 based on the comparison between GDQY and other isolates. Genetic studies of ORFV106 and 107 are reported for the first time in the presented study.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 555, Issue 2, 25 January 2015, Pages 260-268
Journal: Gene - Volume 555, Issue 2, 25 January 2015, Pages 260-268
نویسندگان
Chaohui Duan, Meiying Liao, Han Wang, Xiaohong Luo, Jing Shao, Ying Xu, Wei Li, Wenbo Hao, Shuhong Luo,