کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5905923 | 1159943 | 2014 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Molecular characterization and differential expression of multiple goose dopamine D2 receptors
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کلمات کلیدی
Dopamine D5 receptorDRD3DRD4DRD1DRD2complete coding sequenceDRD5RT-PCRGAPDHORFSNPsInDelJTTcDNA - cDNAAmino acids - اسید آمینه یا آمینو اسیدdifferential expression - بیان دیفرانسیلCloning - تاگسازی یا شبیه سازیAlternative splicing - جابجایی جایگزینBase pair(s) - جفت پایه (ها)open reading frame - قاب خواندن بازUTR یا untranslated regions - منطقه ترجمه نشدهuntranslated region - منطقه غیر ترجمهcomplementary to RNA - مکمل RNAreverse transcription polymerase chain reaction - واکنش زنجیره ای پلیمراز رونویسی معکوسSingle nucleotide polymorphisms - پلیمورفیسم تک نوکلئوتیدیneighbor-joining - پیوستن همسایهCdS - کادمیم سولفید، سولفید کادمیمglyceraldehyde-3-phosphate dehydrogenase - گلیسرالیدید-3-فسفات دهیدروژنازDopamine D4 receptor - گیرنده D4 DopamineDopamine D1 receptor - گیرنده Dopamine D1dopamine D2 receptor - گیرنده دوپامین D2Dopamine D3 receptor - گیرنده دوپامین D3
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
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چکیده انگلیسی
Dopamine D2 receptor (DRD2) gene, a member of the dopamine receptors gene family, has been studied as a candidate gene for broodiness due to its special effects on avian prolactin secretion. Here, the genomic DNA and cDNA sequences of goose (Anser cygnoides) DRD2 gene were cloned and characterized for the first time. The goose DRD2 cDNA is 1353Â bp in length and encodes a protein of 450 amino acids. The length of goose DRD2 genomic DNA is 8350Â bp, including seven exons and six introns. We identified four goose DRD2 variants, which were generated due to alternative splicing. Bioinformatics analysis indicates that all the deduced DRD2 amino acid sequences contain seven putative transmembrane domains and four potential N-glycosylation sites. A phylogenetic tree based on amino acid sequences displays that the goose DRD2 protein is closely related to those of avian species. Semi-quantitative RT-PCR analysis demonstrates that the DRD2-1, DRD2-2 and DRD2-4 transcripts are differentially expressed in the pituitary, ovary, hypothalamus, as well as in the kidney, whereas the DRD2-3 transcript is widely expressed in all the examined tissues at different levels. Meanwhile, 54 single nucleotide polymorphisms (SNPs) and 4 insert-deletion (indel) variations were identified in the coding region and partial intron region of the goose DRD2 gene. Those findings will help us gain insight into the functions of the DRD2 gene in geese.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 535, Issue 2, 10 February 2014, Pages 177-183
Journal: Gene - Volume 535, Issue 2, 10 February 2014, Pages 177-183
نویسندگان
Cui Wang, Yi Liu, Huiying Wang, Huali Wu, Shaoming Gong, Weihu Chen, Daqian He,