Genome-wide SNP discovery and identification of QTL associated with agronomic traits in oil palm using genotyping-by-sequencing (GBS)

- We have used the genotyping-by-sequencing (GBS) approach to identify single nucleotide polymorphisms (SNPs) in oil palm.
- A total of 21,471 SNPs were identified from reduced representation libraries of 108 F2 progeny.
- We constructed a linkage map containing 1085 markers distributed over 17 linkage groups.
- Three quantitative trait loci (QTL) affecting trunk height and a single QTL associated with bunch weight were identified.

Oil palm has become one of the most important oil crops in the world. Marker-assisted selections have played a pivotal role in oil palm breeding programs. Here, we report the use of genotyping-by-sequencing (GBS) approach for a large-scale SNP discovery and genotyping of a mapping population. Reduced representation libraries of 108 F2 progeny were sequenced and a total of 524 million reads were obtained. We detected 21,471 single nucleotide substitutions, most of which (62.6%) represented transition events. Of 3417 fully informative SNP markers, we were able to place 1085 on a linkage map, which spanned 1429.6 cM and had an average of one marker every 1.26 cM. Three QTL affecting trunk height were detected on LG 10, 14 and 15, whereas a single QTL associated with fruit bunch weight was identified on LG 3. The use of GBS approach proved to be rapid, cost-effective and highly reproducible in this species.