کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5907902 | 1160880 | 2014 | 6 صفحه PDF | دانلود رایگان |
- The transcriptome of the Ophiocordyceps sinensis fruiting body was sequenced using 454 technology.
- Most of the genes involved in fruiting body development were screened.
- All the enzymes involved in a putative biosynthesis pathway of cordycepin were discovered.
Ophiocordyceps sinensis is a highly valuable and popular medicinal fungus used as a tonic and roborant for thousands of years in traditional Asian medicine. However, unsustainable harvesting practices have endangered this species and very little is known about its developmental programming, its biochemistry and genetics. To begin to address this, the transcriptome of the medicinal O. sinensis fruiting body was analyzed by high-throughput. In this O. sinensis 454-EST dataset, four mating type genes and 121 genes that may be involved in fruiting body development, especially in signal transduction and transcription regulation, were discovered. Moreover, a model was developed for the synthesis of the primary medicinal compound, cordycepin, and the putative biosynthetic enzymes identified. This transcriptome dataset provides a significant new resource for gene discovery in O. sinensis and dissection of its valuable biosynthetic and developmental pathways.
Journal: Genomics - Volume 103, Issue 1, January 2014, Pages 154-159